| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.226418 |
| Chromosome: | chromosome_12 |
| Location: | 6206648 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g536600 | FAP78 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCCAACATGGACATGAGGAAGACGAGAGC |
| Internal bar code: | GGGTACGTTCGCCTCGAGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 403 |
| LEAP-Seq percent confirming: | 93.8285 |
| LEAP-Seq n confirming: | 2691 |
| LEAP-Seq n nonconfirming: | 177 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTTGCTTACGAGCACACA |
| Suggested primer 2: | GATGATGGACCCCATACAGG |