| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.226427 |
| Chromosome: | chromosome 10 |
| Location: | 2931043 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440350 | (1 of 3) PF07887 - Calmodulin binding protein-like (Calmodulin_bind) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACAGGCGGGGCGGTCTGAAGGGCGCGC |
| Internal bar code: | CAGGTCGCATAGATGGATGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 437 |
| LEAP-Seq percent confirming: | 99.1845 |
| LEAP-Seq n confirming: | 973 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAACCCATTGAACTGGACT |
| Suggested primer 2: | CACATCAGTACCCGCACATC |