| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.226430 |
| Chromosome: | chromosome 17 |
| Location: | 857753 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g702351 | HLM26 | putative N-methyltransferase; (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAACTCGCAACTAACGCCTCTGCCTATGC |
| Internal bar code: | CAATCTTCTCCCCAAAAACCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 526 |
| LEAP-Seq percent confirming: | 99.2896 |
| LEAP-Seq n confirming: | 4612 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGACGGCTGCTACCTTTTC |
| Suggested primer 2: | GTCAGCACGATTTCAGACGA |