Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.226431 |
Chromosome: | chromosome 14 |
Location: | 1981863 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g621351 | (1 of 2) 2.7.6.5 - GTP diphosphokinase / Stringent factor | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGTTGAGTGGCAGCGGGGAGGGTGGAT |
Internal bar code: | TGATTTTAGGTCTTATACTGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 269 |
LEAP-Seq percent confirming: | 99.0826 |
LEAP-Seq n confirming: | 2376 |
LEAP-Seq n nonconfirming: | 22 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGCTGGAATGAGAGGATGG |
Suggested primer 2: | CGACGAATGCTGTCAAGTGT |