| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.226435 |
| Chromosome: | chromosome 10 |
| Location: | 5447081 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458550 | STIC2 | Chloroplast protein biogenesis factor; (1 of 1) PTHR33449:SF1 - NUCLEOID-ASSOCIATED PROTEIN YBAB | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTGCTTGGATGCCTGCGGGCTTGGATAC |
| Internal bar code: | GGCCAAGCTACGGGCAGGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 487 |
| LEAP-Seq percent confirming: | 99.4166 |
| LEAP-Seq n confirming: | 852 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCAGGGCTGTAAGGGCTA |
| Suggested primer 2: | TTTTGGACTGTGTGGCATGT |