| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.226460 |
| Chromosome: | chromosome 8 |
| Location: | 4601263 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g383200 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGGCGCAGGCGTGCAGATGTTCTGGTG |
| Internal bar code: | ATCCGGCCCTGGTGCCACTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 594 |
| LEAP-Seq percent confirming: | 95.9906 |
| LEAP-Seq n confirming: | 4884 |
| LEAP-Seq n nonconfirming: | 204 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCAAAGCTGAGGACGAT |
| Suggested primer 2: | AGGACCTCATGTCGTCCATC |