| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.226472 |
| Chromosome: | chromosome 12 |
| Location: | 4709944 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523600 | (1 of 3) PTHR13367//PTHR13367:SF8 - TUMOR NECROSIS FACTOR-RELATED // OTU DOMAIN-CONTAINING PROTEIN 7B | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCTTGGCTGCCTGTTCCTTCCGCCGGCC |
| Internal bar code: | AAGGAGTTCAAAGGCGGCGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 369 |
| LEAP-Seq percent confirming: | 99.4924 |
| LEAP-Seq n confirming: | 1176 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTAACACGTGGAGAGACA |
| Suggested primer 2: | TCTTCGCCAGGTATAACGCT |