| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.226495 |
| Chromosome: | chromosome 3 |
| Location: | 4172654 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173750 | POB19 | Proteome of basal body 19; (1 of 80) IPR003882 - Pistil-specific extensin-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCAACCGAGCTTGCGCGCTCGCTCCAGC |
| Internal bar code: | CTTTAAATCCCATCTTTCCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 544 |
| LEAP-Seq percent confirming: | 92.8767 |
| LEAP-Seq n confirming: | 1356 |
| LEAP-Seq n nonconfirming: | 104 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTAGCCAGGTTTTGCGATG |
| Suggested primer 2: | GCTTTGAGCCTGTCCACTTC |