| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.226503 |
| Chromosome: | chromosome_10 |
| Location: | 6537654 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g467000 | IFT55,IFT57 | Intraflagellar transport protein 57 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCACACCGGTGCGGGCACGCGTGCCGTGT |
| Internal bar code: | ATTGACCATCGCTGATAGAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 572 |
| LEAP-Seq percent confirming: | 93.4953 |
| LEAP-Seq n confirming: | 1193 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGACAGAGGGCATGTGTG |
| Suggested primer 2: | GTGGACAAGGCACGGTATCT |