| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.226508 |
| Chromosome: | chromosome 7 |
| Location: | 207699 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313600 | CGL33A,SMM21 | (1 of 1) PTHR12176//PTHR12176:SF29 - UNCHARACTERIZED // S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN; conserved methyltransferase of unknown function | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCTTTGCAAGTGCTTACTTACCACCTC |
| Internal bar code: | CCTAGCTCCCAGCGTCGCTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 518 |
| LEAP-Seq percent confirming: | 99.6176 |
| LEAP-Seq n confirming: | 11202 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTTACGCACCCTTGTTGG |
| Suggested primer 2: | CTACCGGCGCAGAATATAGC |