| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.226546 |
| Chromosome: | chromosome 16 |
| Location: | 527723 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692650 | CGL4 | Conserved in the Green Lineage; (1 of 173) IPR029058 - Alpha/Beta hydrolase fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGGGATTTTCTGTTGGGCGCCACAGGG |
| Internal bar code: | TGGAGTCGGCGTCTTCTCAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 173 |
| LEAP-Seq percent confirming: | 99.3671 |
| LEAP-Seq n confirming: | 157 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGACACCCACACACACTG |
| Suggested primer 2: | ACTTGCAAGGGGGTAAGGTT |