| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.226549 |
| Chromosome: | chromosome 7 |
| Location: | 4125434 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342800 | CGL16 | (1 of 1) PF07063 - Domain of unknown function (DUF1338) (DUF1338); Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGGCAACAACCCCCTGCGAGCCGCCTT |
| Internal bar code: | GGTGGGTATCGGGAGGAGCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 407 |
| LEAP-Seq percent confirming: | 99.4581 |
| LEAP-Seq n confirming: | 2753 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCCGCATCTCAGTACCAT |
| Suggested primer 2: | GTTTGCCTGTTTGGGTGTTT |