| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.226552 |
| Chromosome: | chromosome 14 |
| Location: | 1673986 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619200 | (1 of 140) IPR002048 - EF-hand domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTAACTCGCTCACTCACCCCTTCACATA |
| Internal bar code: | CGTCCTGCCCTGATTGACATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 475 |
| LEAP-Seq percent confirming: | 99.1597 |
| LEAP-Seq n confirming: | 236 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGACTTGGCAGCAGAGGTC |
| Suggested primer 2: | ACTGCTTCAATCTCCTGCGT |