Insertion junction: LMJ.RY0402.226559_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.226559
Chromosome:chromosome 6
Location:2192236
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g265950 DHC3 Axonemal Inner Arm Dynein Heavy Chain 3, pseudogene; (1 of 2) PTHR10676:SF136 - DYNEIN HEAVY CHAIN 6, AXONEMAL CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCATCTACCTGCGCCACGCCCACGCCGA
Internal bar code:GCACAGGAGGGTTAGCTGAGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:617
LEAP-Seq percent confirming:98.4253
LEAP-Seq n confirming:8563
LEAP-Seq n nonconfirming:137
LEAP-Seq n unique pos:105

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGCTCGAGGGCTTCTATG
Suggested primer 2:GCCAAGCAAGCTAGTAACGG