Insertion junction: LMJ.RY0402.226572_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.226572
Chromosome:chromosome 6
Location:4204768
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g278234 FAP343 (1 of 19) PF00397 - WW domain (WW); Flagellar Associated Protein 343 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCACTGCACCCTCGCCTTCGCCCGCCCTG
Internal bar code:AGCTGTAAGCGGTTCTCCCAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1
LEAP-Seq percent confirming:81.1594
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AACACGACACAAGCACAAGC
Suggested primer 2:CTGGAGCTTCTGGTCCTTTG