| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.226603 |
| Chromosome: | chromosome 12 |
| Location: | 72433 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g483800 | CPY | Serine carboxypeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGATTCCTTTGCAGCCTCCGCCCTCGCA |
| Internal bar code: | AATTCTAGACGTGCCGCGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 394 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 169 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCGGAACACCGCTAATTC |
| Suggested primer 2: | AGGTATCAACCCCACCCTTC |