| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.226668 |
| Chromosome: | chromosome 5 |
| Location: | 1023292 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246552 | (1 of 2) PTHR10566//PTHR10566:SF86 - CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED // ABC1 PROTEIN KINASE 6 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAATCATGAATGCACCCCCCCCTATCTCC |
| Internal bar code: | GATGCAGGTCTAAATTTGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 205 |
| LEAP-Seq percent confirming: | 54.717 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACACGCTCAACGACTCC |
| Suggested primer 2: | AGGTGTGTGTGTGGGGAAGT |