| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.226676 |
| Chromosome: | chromosome_2 |
| Location: | 4261236 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g098900 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCAGCAAAACCTGGAGCTGGCCGATGAAGT |
| Internal bar code: | GGAGGCCATCGGATAGCGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 439 |
| LEAP-Seq percent confirming: | 93.8967 |
| LEAP-Seq n confirming: | 200 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGATGTCTGCGAGCTTGT |
| Suggested primer 2: | ACGCTGAGGGATTGCTTATG |