| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.226724 |
| Chromosome: | chromosome 3 |
| Location: | 4853396 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179150 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCCCGACCACGGCCCAACCACGGCTCAC |
| Internal bar code: | TCGGGAGGTGATCGTGAGATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1072 |
| LEAP-Seq percent confirming: | 44.3188 |
| LEAP-Seq n confirming: | 2586 |
| LEAP-Seq n nonconfirming: | 3249 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGCGGTGTAATGCGATG |
| Suggested primer 2: | GCAATCTCGCAATTACCCAT |