| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.226762 |
| Chromosome: | chromosome 6 |
| Location: | 7916564 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g303350 | FAL9 | Conserved protein of unknown function | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCGCGACTTGCGCCAGGTATGCGCTAC |
| Internal bar code: | GGTGGGACCGGGGCCGGCGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 479 |
| LEAP-Seq percent confirming: | 89.9811 |
| LEAP-Seq n confirming: | 476 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCACACACACACACACGTA |
| Suggested primer 2: | GCGTGATTACCGAGGTGTTT |