| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.226763 |
| Chromosome: | chromosome 6 |
| Location: | 2507619 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g269050 | TIC62,YCF39-2 | (1 of 2) PTHR14194:SF71 - PRESTALK A DIFFERENTIATION PROTEIN A; Putative dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGGCGTATGGCAAGCGTGGGCCGTGTCA |
| Internal bar code: | GTGGGTGTTAAACTCGTCTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 457 |
| LEAP-Seq percent confirming: | 99.0535 |
| LEAP-Seq n confirming: | 4500 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACTGCATCACAACACCCG |
| Suggested primer 2: | GTGCAAGCAGACCTGCATAA |