| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.226794 |
| Chromosome: | chromosome 14 |
| Location: | 3118125 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g628702 | (1 of 1) PF11919 - Domain of unknown function (DUF3437) (DUF3437) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATGTCAGGAAATTGGCGCAGGAGAGGGC |
| Internal bar code: | CTACAGTCATTTGATGAGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 464 |
| LEAP-Seq percent confirming: | 98.7585 |
| LEAP-Seq n confirming: | 875 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | TTGGACGAGCTCTCACTCCT |