| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.226797 |
| Chromosome: | chromosome 4 |
| Location: | 2288668 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220350 | ATPVA3 | Vacuolar ATP synthase subunit A; (1 of 3) K02154 - V-type H+-transporting ATPase subunit a (ATPeV0A, ATP6N) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAGTCCCCCTCCCCACCCCAACAATGC |
| Internal bar code: | GATATCGTAGTAGGGACGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 133 |
| LEAP-Seq percent confirming: | 10.7287 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 441 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCATAAGAATGGATGGTG |
| Suggested primer 2: | TCGGTTGTCCGTCTCCTATC |