| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.226809 |
| Chromosome: | chromosome 3 |
| Location: | 8165695 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200400 | (1 of 1) PTHR30383 - PERIPLASMIC BROAD-SPECIFICITY ESTERASE/LIPASE/PROTEASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCCGAAACCCAAATTGCGGGTGCTGTT |
| Internal bar code: | GAGCATCCTTGGACGTCTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1113 |
| LEAP-Seq percent confirming: | 98.3839 |
| LEAP-Seq n confirming: | 6879 |
| LEAP-Seq n nonconfirming: | 113 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTCTAGCGTTCGGCGACT |
| Suggested primer 2: | CGTCATCAAGGTCAGCAGAA |