| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.226832 |
| Chromosome: | chromosome 8 |
| Location: | 4963451 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g385650 | (1 of 4) K02206 - cyclin-dependent kinase 2 (CDK2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGGGAGGTCACTGACACGGACCCAGAC |
| Internal bar code: | ACATAGCGTGGGTGGCGGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 451 |
| LEAP-Seq percent confirming: | 99.5434 |
| LEAP-Seq n confirming: | 3488 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTATTTGCCTGAGCCTGAC |
| Suggested primer 2: | TGTGTGTGTGTGTGTGGAGG |