| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.226852 |
| Chromosome: | scaffold 18 |
| Location: | 187901 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749497 | (1 of 1) K01945 - phosphoribosylamine--glycine ligase (purD) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAACAGCATAGCTGGACACGGAATACTG |
| Internal bar code: | AACTCAGTTTATAAAGGTGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 712 |
| LEAP-Seq percent confirming: | 96.6165 |
| LEAP-Seq n confirming: | 257 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTTTCCCATGCGATTTT |
| Suggested primer 2: | GCTGGGAGGGAAGTAGAACC |