Insertion junction: LMJ.RY0402.226870_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.226870
Chromosome:chromosome 16
Location:1440536
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g652400 FAL18,FAP183 (1 of 17) PF13202 - EF hand (EF-hand_5); EF-hand domain Flagellar Associated Protein 183 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCATTCCACTGACCACACAAACGCGCACA
Internal bar code:TTTAGTACGAGACTTTCAATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:551
LEAP-Seq percent confirming:98.8935
LEAP-Seq n confirming:715
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTAGGCTGTGTGTGAGGC
Suggested primer 2:GGGTTGAAAGGCTGTTGGTA