| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.226875 |
| Chromosome: | chromosome 7 |
| Location: | 1819448 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325728 | (1 of 5) PF12681 - Glyoxalase-like domain (Glyoxalase_2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTTCAATGTCACGCCGGTGTTGTGAAGC |
| Internal bar code: | AGATGCCTTGGTTAGCCGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 568 |
| LEAP-Seq percent confirming: | 98.0961 |
| LEAP-Seq n confirming: | 1082 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGCAGCATCAGCATTTG |
| Suggested primer 2: | TATTCGCAGCAACTGAATGC |