Insertion junction: LMJ.RY0402.226875_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.226875
Chromosome:chromosome 7
Location:1819452
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g325728 (1 of 5) PF12681 - Glyoxalase-like domain (Glyoxalase_2) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCGACCGAAGCGTGGATTCCTCGAGACT
Internal bar code:CCGGACTAAACCGTGTGTGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:899
LEAP-Seq percent confirming:99.3857
LEAP-Seq n confirming:1618
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AACAGCAGCATCAGCATTTG
Suggested primer 2:TATTCGCAGCAACTGAATGC