Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.226880 |
Chromosome: | chromosome 4 |
Location: | 1870063 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre04.g217850 | (1 of 3) IPR000104//IPR003439//IPR003593//IPR013525//IPR027417 - Antifreeze protein, type I // ABC transporter-like // AAA+ ATPase domain // ABC-2 type transporter // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTCGTTGGCAATCACCCCCAAAACATAT |
Internal bar code: | GCGCATACGCAAAGGCACCGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 347 |
LEAP-Seq percent confirming: | 94.902 |
LEAP-Seq n confirming: | 242 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCGTAGGACAGTGTATGCGA |
Suggested primer 2: | AGTGTGTTCGTTCCACACCA |