| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.226890 |
| Chromosome: | chromosome 1 |
| Location: | 472289 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g002750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTCACTACCACCCTTGATCCGGAATAC |
| Internal bar code: | AGTGCGTTGCACGCCTGGCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 577 |
| LEAP-Seq percent confirming: | 98.5178 |
| LEAP-Seq n confirming: | 997 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTAGGCATGTGACGAAG |
| Suggested primer 2: | CCGTCCATGTAAAACGGAGT |