| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.226908 |
| Chromosome: | chromosome 7 |
| Location: | 5185512 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348550 | TGL13 | (1 of 3) PTHR21493:SF153 - PROTEIN T08B1.4, ISOFORM B-RELATED; Putative triacylglycerol lipase | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCAGTGCCCTACACTGGCTATTGAGGC |
| Internal bar code: | TGGCCTGGCAGCAAGTAGAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 401 |
| LEAP-Seq percent confirming: | 99.1576 |
| LEAP-Seq n confirming: | 1648 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCACCTCACCACACCTC |
| Suggested primer 2: | GTAGAGGCAGAGGGGAGAGG |