| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.226910 |
| Chromosome: | chromosome 17 |
| Location: | 5575688 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g737650 | SOM6 | (1 of 1) IPR000104//IPR007213//IPR011610//IPR029063 - Antifreeze protein, type I // Leucine carboxyl methyltransferase // S-adenosyl-L-methionine-dependent methyltransferase, putative // S-adenosyl-L-methionine-dependent methyltransferase; S-adenosyl-L-methionine-dependent O-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCCTAAACCATATGGTAATCACAAATG |
| Internal bar code: | GTGGCAATTGCGATCGTGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 638 |
| LEAP-Seq percent confirming: | 99.1981 |
| LEAP-Seq n confirming: | 1237 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAACAGCTGGAAGAAGGC |
| Suggested primer 2: | GCCCACACACACCTACACAC |