| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.226912 |
| Chromosome: | chromosome_14 |
| Location: | 770948 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g612950 | CYG17 | Adenylate/guanylate cyclase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTGAGGCCGAACTGCGAGGCCGAGGCCCT |
| Internal bar code: | GCTTACGAACGCGGCTTGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 144 |
| LEAP-Seq percent confirming: | 50.3759 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACGGCGGATACACAAAGC |
| Suggested primer 2: | CCTTAGCTGCCTTGCGTTAC |