| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.226933 |
| Chromosome: | chromosome 1 |
| Location: | 5355798 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037550 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACTGAAATGGTGGTGATTACGCCGGCC |
| Internal bar code: | AAAACTAATCAGTGTTCGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 613 |
| LEAP-Seq percent confirming: | 99.7766 |
| LEAP-Seq n confirming: | 2233 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTTCACGTGGATGGTGAC |
| Suggested primer 2: | TCAGGTACACGATGCCTGAC |