| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.226946 |
| Chromosome: | chromosome_10 |
| Location: | 5343957 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g458050 | BCA3 | Branched chain amino acid aminotransferase | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTACGCATGTCTGCGGCTGCATGGCAATG |
| Internal bar code: | AGTCCTTCGGGCTCTGATTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 113 |
| LEAP-Seq percent confirming: | 43.6866 |
| LEAP-Seq n confirming: | 474 |
| LEAP-Seq n nonconfirming: | 611 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAACGGACCAGACCTGTG |
| Suggested primer 2: | ATGTATGTGCGTGCGTGAAT |