Insertion junction: LMJ.RY0402.226946_4

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.226946
Chromosome:chromosome 13
Location:3741534
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTCACCGCCCTCGACGAACACGCCACAAG
Internal bar code:TGTGTTCCCGCTCATTTTCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:587
LEAP-Seq percent confirming:99.0511
LEAP-Seq n confirming:5637
LEAP-Seq n nonconfirming:54
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AACATGCCTGGTTGTAAGCC
Suggested primer 2:GAGGTAGCTGAACTCCACCG