| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.226949 |
| Chromosome: | chromosome 12 |
| Location: | 3332883 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497652 | (1 of 8) PTHR10366//PTHR10366:SF411 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCACACGTACGGAGGCGACAGGTGGGG |
| Internal bar code: | GTTCGCTAGCATGCAAGGGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 603 |
| LEAP-Seq percent confirming: | 95.5085 |
| LEAP-Seq n confirming: | 13609 |
| LEAP-Seq n nonconfirming: | 640 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGAGTCCTAGCCAAGCC |
| Suggested primer 2: | GTGTGTGTGTGCGTGTGTGT |