Insertion junction: LMJ.RY0402.226952_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.226952
Chromosome:chromosome 17
Location:6649231
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g743847 (1 of 1) IPR000504//IPR003613 - RNA recognition motif domain // U box domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGGGCTGGCTTTTCGTCTCAGGATTCGG
Internal bar code:GGGGGATCCCGTACGTAATGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:542
LEAP-Seq percent confirming:40.9457
LEAP-Seq n confirming:1576
LEAP-Seq n nonconfirming:2273
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:ATCTGGTGGTGATGCTAGGG
Suggested primer 2:CTCGTACGTGTTGCCTGTGT