Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.226958 |
Chromosome: | chromosome 2 |
Location: | 3269266 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g095080 | (1 of 1) IPR001107//IPR002499 - Band 7 protein // Major vault protein, N-terminal | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCACTGTCTCACAGATTGTCTCACTTGA |
Internal bar code: | GGTTGGGCGTCGTTCTTTTGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 424 |
LEAP-Seq percent confirming: | 98.9922 |
LEAP-Seq n confirming: | 3438 |
LEAP-Seq n nonconfirming: | 35 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTGTAGGAGGTGTGAGGGA |
Suggested primer 2: | CGAGCTATGTACCCACGGTT |