Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.226974 |
Chromosome: | chromosome 1 |
Location: | 4291430 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g028850 | (1 of 25) PF00581 - Rhodanese-like domain (Rhodanese) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTAGGACCGTATCCGGTACATGTCAGTA |
Internal bar code: | ATCGGTGGCTACGCCTGATGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 494 |
LEAP-Seq percent confirming: | 98.7907 |
LEAP-Seq n confirming: | 3758 |
LEAP-Seq n nonconfirming: | 46 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGATGTGATGTCGTGCGTT |
Suggested primer 2: | CTTCCGACTTTGCCAGTCTC |