Insertion junction: LMJ.RY0402.226974_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.226974
Chromosome:chromosome 1
Location:4291430
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g028850 (1 of 25) PF00581 - Rhodanese-like domain (Rhodanese) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTAGGACCGTATCCGGTACATGTCAGTA
Internal bar code:ATCGGTGGCTACGCCTGATGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:494
LEAP-Seq percent confirming:98.7907
LEAP-Seq n confirming:3758
LEAP-Seq n nonconfirming:46
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ATGATGTGATGTCGTGCGTT
Suggested primer 2:CTTCCGACTTTGCCAGTCTC