Insertion junction: LMJ.RY0402.227015_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.227015
Chromosome:chromosome 3
Location:4122068
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g173250 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGCGGACACAGGGCAGGGTGTCGGGTGT
Internal bar code:CTCTGCCCAGGGATGAGTCCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:712
LEAP-Seq percent confirming:99.2806
LEAP-Seq n confirming:1104
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGGTCTTGCAGTCAATCA
Suggested primer 2:AGCCCAGTCTTCTAGCACCA