| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227017 |
| Chromosome: | chromosome 13 |
| Location: | 2508816 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580350 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAGTATTGTAAAGATGACCACGCTGTTA |
| Internal bar code: | CTACCGGTGACGGCGACGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 713 |
| LEAP-Seq percent confirming: | 99.0426 |
| LEAP-Seq n confirming: | 2276 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGGACCCGTTTTGTAGT |
| Suggested primer 2: | ATAGCGAACGAGATGAGCGT |