| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227026 |
| Chromosome: | chromosome 5 |
| Location: | 1117534 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245800 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGAGCCAGCCGCAAAGCGACTTACAAAC |
| Internal bar code: | GGAGGGGCCGTAATGGTTAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 473 |
| LEAP-Seq percent confirming: | 74.7733 |
| LEAP-Seq n confirming: | 1402 |
| LEAP-Seq n nonconfirming: | 473 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCTGCCGTTGTGCTTCTG |
| Suggested primer 2: | GAGCACGGTAAGAGGAGCAC |