Insertion junction: LMJ.RY0402.227038_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.227038
Chromosome:chromosome 3
Location:1963196
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g155700 (1 of 2) PTHR22840//PTHR22840:SF12 - FAMILY NOT NAMED // WD REPEAT-CONTAINING PROTEIN 36 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTCCGCCTTGTTGTACGAGAGGTCGACG
Internal bar code:TAGAAATCATCATAGTGAAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:622
LEAP-Seq percent confirming:99.1257
LEAP-Seq n confirming:2721
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:77

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTGGTTCAAAAATGCCAC
Suggested primer 2:ACCAATCTGACCTTTGTGGC