| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.227063 |
| Chromosome: | chromosome 10 |
| Location: | 3964757 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448600 | (1 of 1) PTHR20956:SF0 - KIF1-BINDING PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAACCCCACACCCCACTCCCCAACCCTT |
| Internal bar code: | ACTGTAAATTGATCTGAACCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 332 |
| LEAP-Seq percent confirming: | 21.1921 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 119 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGCCATGAGGAAGTAGG |
| Suggested primer 2: | ATGCCGTTCTTCAAGGTGAC |