| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.227064 |
| Chromosome: | chromosome 11 |
| Location: | 2513917 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475950 | HLM16 | putative N-methyltransferase; (1 of 5) IPR001214//IPR003616 - SET domain // Post-SET domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCCTGCTCGGTCTCAGCCTCGCCAGTTT |
| Internal bar code: | CGAGAGCGTTCACCGTCCTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 609 |
| LEAP-Seq percent confirming: | 99.5669 |
| LEAP-Seq n confirming: | 5058 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACCATTGAAGGAGCCAC |
| Suggested primer 2: | GTGGTACTCCCGATGCAAGT |