| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227066 |
| Chromosome: | chromosome 3 |
| Location: | 7749964 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g203451 | CYA5 | Adenylate cyclase; (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCATCGCGGAGGCTGTCTCCATCGCCGCC |
| Internal bar code: | ACTGAGTGCCGAGTGGCGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 314 |
| LEAP-Seq percent confirming: | 98.4556 |
| LEAP-Seq n confirming: | 255 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGATCGAACCCTACACCA |
| Suggested primer 2: | TGTAAGCCTCACCTTCCTCG |