| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.227129 |
| Chromosome: | chromosome 2 |
| Location: | 261217 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g074900 | (1 of 1) PTHR10774//PTHR10774:SF82 - EXTENDED SYNAPTOTAGMIN-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCTACGGGCAGGGTGGCACTGTGCGCC |
| Internal bar code: | GGCGCGGCGCCGCCTTTTGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 273 |
| LEAP-Seq percent confirming: | 97.2569 |
| LEAP-Seq n confirming: | 390 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAAGCTTTAGCGTGGGTC |
| Suggested primer 2: | ACAGCATACGGTAACCCTGC |