| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.227134 |
| Chromosome: | chromosome 12 |
| Location: | 9654707 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553678 | (1 of 3) PTHR17130:SF24 - GAN | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTCCCCACCCACACGCACACGCATGTAT |
| Internal bar code: | TTATAAGGATGTCCGCTGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 756 |
| LEAP-Seq percent confirming: | 89.2839 |
| LEAP-Seq n confirming: | 5274 |
| LEAP-Seq n nonconfirming: | 633 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTTGGTTGAGCACATGG |
| Suggested primer 2: | TAAGGGGTAAGGCAGGGAGT |